Journal: ChemistryOpen

Article Title: Phytochemical Profiling of the Halophyte Artemisia fukudo Makino and Its In Vitro Effects on Androgen‐Related Pathways Associated With Benign Prostatic Hyperplasia and Alopecia

doi: 10.1002/open.202500481

Figure Lengend Snippet: The effects of 90% MeOH fraction of A. fukudo (AFE) on the expressions of androgen receptor (AR), prostate‐specific antigen (PSA), and 5‐alpha reductase type 2 (5αR2) in LNCaP cells activated with TP (testosterone propionate). Cells were treated with TP (0.5 μM) and then treated with AFE (6.25, 12.5, and 25 μg/mL) or finasteride (10 μM) for 72 h. The expression levels of AR, 5αR2, and PSA in cells were analyzed by Western blotting. Band intensities were quantified by densitometry, normalized to α‐tubulin, and expressed relative to the TP‐treated group (set to 1). Data are presented as the mean ± SD (error bars) from three independent experiments ( n = 3). Statistical analysis was performed by one‐way ANOVA followed by Duncan's multiple range test; * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to TP‐treated cells. Fina, finasteride; NC, nontreated control; TP, testosterone propionate.

Article Snippet: RWPE‐1 cell (human prostatic epithelial cell line; RRID: CVCL‐3791) and LNCaP cell (human prostate adenocarcinoma cell line; RRID: CVCL‐0395) were purchased from American Type Culture Collection (ATCC, Manassas, VA, United States; CRL‐11609D and CRL‐1740).

Techniques: Expressing, Western Blot, Control